Introduction: In this study, it was investigated whether dardarin (LRRK2) and isthmin-1 (ISM1) play a role in the protective effect of hydroxytyrosol (HT) used to prevent liver damage caused by corn syrup in rats.

Methods: Rats were divided into four groups with six in each group: 1) control, 2) HT, 3) corn syrup, and 4) corn syrup + HT. Rats were given water containing 30% corn syrup for six weeks. At the same time, HT-containing liquid was given orally at 4 ml/kg/day, alone and together with corn syrup for six weeks. The weights of the rats were measured every week. LRRK2 and ISM1 molecules in liver tissue were evaluated by histopathological methods. Biochemical parameters were also examined with the enzyme-linked immunosorbent assay (ELISA) method.

Results: It was found that weight gain was less in rats receiving HT than in those consuming corn syrup. The increase in cholesterol, triglyceride, and liver enzyme levels because of corn syrup consumption decreased with HT consumption. As a result of histopathological analysis, it was observed that the increase in LRRK2 and ISM1 levels observed in the liver tissue in the corn syrup-administered group decreased when HT was administered together with corn syrup. In addition, it was determined that the increase in sinusoidal expansion and hepatocyte necrosis observed in the liver tissue as a result of corn syrup application decreased as a result of the application of HT together with corn syrup.

Conclusion: The protective effect of HT against the damage caused by corn syrup in the liver has been demonstrated once again; however, LRRK2 and ISM1 are thought to contribute to this issue.